Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected 111In-labeled human mesenchymal stromal cells.
Identifieur interne : 001765 ( Main/Exploration ); précédent : 001764; suivant : 001766Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected 111In-labeled human mesenchymal stromal cells.
Auteurs : RBID : pubmed:19921546English descriptors
- KwdEn :
- Animals, Cell Death, Cells, Cultured, Chromosomes, Human, Y, Female, Heart (radionuclide imaging), Humans, In Situ Hybridization, Fluorescence, Inflammation (pathology), Inflammation (radionuclide imaging), Injections, Intramuscular, Male, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells (pathology), Mesenchymal Stromal Cells (radionuclide imaging), Myocardium (pathology), Organometallic Compounds, Pilot Projects, Radiopharmaceuticals, Staining and Labeling (methods), Swine, Time Factors, Transplantation, Heterologous, Tropolone (analogs & derivatives), X Chromosome.
- MESH :
- chemical , analogs & derivatives : Tropolone.
- chemical : Organometallic Compounds, Radiopharmaceuticals.
- methods : Staining and Labeling.
- pathology : Inflammation, Mesenchymal Stromal Cells, Myocardium.
- radionuclide imaging : Heart, Inflammation, Mesenchymal Stromal Cells.
- Animals, Cell Death, Cells, Cultured, Chromosomes, Human, Y, Female, Humans, In Situ Hybridization, Fluorescence, Injections, Intramuscular, Male, Mesenchymal Stem Cell Transplantation, Pilot Projects, Swine, Time Factors, Transplantation, Heterologous, X Chromosome.
Abstract
This pilot trial aimed to investigate the utilization of (111)In-labeling of mesenchymal stromal cells (MSC) for in vivo tracking after intramyocardial transplantation in a xenotransplantation model with gender mismatched cells. Human male MSC were expanded ex vivo and labeled with (111)In-tropolone. Ten female pigs were included. The labeled cells were transplanted intramyocardially using a percutaneous injection system. The (111)In activity was determined using gamma camera imaging. Excised hearts were analyzed by fluorescence in situ hybridization (FISH) and microscopy. Gamma camera imaging revealed focal cardiac (111)In accumulations up to 6 days after injection (N = 4). No MSC could be identified with FISH, and microscopy identified widespread acute inflammation. Focal (111)In accumulation, inflammation but no human MSC were similarly seen in pigs (N = 2) after immunosuppression. A comparable retention of (111)In activity was observed after intramyocardial injection of (111)In-tropolone (without cells) (N = 2), but without sign of myocardial inflammation. Injection of labeled non-viable cells (N = 1) also led to high focal (111)In activity up to 6 days after intramyocardial injection. As a positive control of the FISH method, we identified labeled cells both in culture and immediately after cell injection in one pig. This pilot trial suggests that after intramyocardial injection (111)In stays in the myocardium despite possible disappearance of labeled cells. This questions the clinical use of (111)In-labeled cells for tracking. The results further suggest that xenografting of human MSC into porcine hearts leads to inflammation contradicting previous studies implying a special immunoprivileged status for MSC.
DOI: 10.1007/s10554-009-9532-4
PubMed: 19921546
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected 111In-labeled human mesenchymal stromal cells.</title><author><name sortKey="Lyngbaek, Stig" uniqKey="Lyngbaek S">Stig Lyngbaek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cardiology, The Heart Centre, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Cardiology, The Heart Centre, Copenhagen University Hospital Rigshospitalet, Copenhagen</wicri:regionArea></affiliation></author><author><name sortKey="Ripa, Rasmus S" uniqKey="Ripa R">Rasmus S Ripa</name></author><author><name sortKey="Haack S Rensen, Mandana" uniqKey="Haack S Rensen M">Mandana Haack-Sørensen</name></author><author><name sortKey="Cortsen, Annette" uniqKey="Cortsen A">Annette Cortsen</name></author><author><name sortKey="Kragh, Linda" uniqKey="Kragh L">Linda Kragh</name></author><author><name sortKey="Andersen, Claus B" uniqKey="Andersen C">Claus B Andersen</name></author><author><name sortKey="J Rgensen, Erik" uniqKey="J Rgensen E">Erik Jørgensen</name></author><author><name sortKey="Kjaer, Andreas" uniqKey="Kjaer A">Andreas Kjaer</name></author><author><name sortKey="Kastrup, Jens" uniqKey="Kastrup J">Jens Kastrup</name></author><author><name sortKey="Hesse, Birger" uniqKey="Hesse B">Birger Hesse</name></author></titleStmt><publicationStmt><date when="2010">2010</date><idno type="doi">10.1007/s10554-009-9532-4</idno><idno type="RBID">pubmed:19921546</idno><idno type="pmid">19921546</idno><idno type="wicri:Area/Main/Corpus">001C18</idno><idno type="wicri:Area/Main/Curation">001C18</idno><idno type="wicri:Area/Main/Exploration">001765</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Cells, Cultured</term><term>Chromosomes, Human, Y</term><term>Female</term><term>Heart (radionuclide imaging)</term><term>Humans</term><term>In Situ Hybridization, Fluorescence</term><term>Inflammation (pathology)</term><term>Inflammation (radionuclide imaging)</term><term>Injections, Intramuscular</term><term>Male</term><term>Mesenchymal Stem Cell Transplantation</term><term>Mesenchymal Stromal Cells (pathology)</term><term>Mesenchymal Stromal Cells (radionuclide imaging)</term><term>Myocardium (pathology)</term><term>Organometallic Compounds</term><term>Pilot Projects</term><term>Radiopharmaceuticals</term><term>Staining and Labeling (methods)</term><term>Swine</term><term>Time Factors</term><term>Transplantation, Heterologous</term><term>Tropolone (analogs & derivatives)</term><term>X Chromosome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Tropolone</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Organometallic Compounds</term><term>Radiopharmaceuticals</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Inflammation</term><term>Mesenchymal Stromal Cells</term><term>Myocardium</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Heart</term><term>Inflammation</term><term>Mesenchymal Stromal Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Cells, Cultured</term><term>Chromosomes, Human, Y</term><term>Female</term><term>Humans</term><term>In Situ Hybridization, Fluorescence</term><term>Injections, Intramuscular</term><term>Male</term><term>Mesenchymal Stem Cell Transplantation</term><term>Pilot Projects</term><term>Swine</term><term>Time Factors</term><term>Transplantation, Heterologous</term><term>X Chromosome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This pilot trial aimed to investigate the utilization of (111)In-labeling of mesenchymal stromal cells (MSC) for in vivo tracking after intramyocardial transplantation in a xenotransplantation model with gender mismatched cells. Human male MSC were expanded ex vivo and labeled with (111)In-tropolone. Ten female pigs were included. The labeled cells were transplanted intramyocardially using a percutaneous injection system. The (111)In activity was determined using gamma camera imaging. Excised hearts were analyzed by fluorescence in situ hybridization (FISH) and microscopy. Gamma camera imaging revealed focal cardiac (111)In accumulations up to 6 days after injection (N = 4). No MSC could be identified with FISH, and microscopy identified widespread acute inflammation. Focal (111)In accumulation, inflammation but no human MSC were similarly seen in pigs (N = 2) after immunosuppression. A comparable retention of (111)In activity was observed after intramyocardial injection of (111)In-tropolone (without cells) (N = 2), but without sign of myocardial inflammation. Injection of labeled non-viable cells (N = 1) also led to high focal (111)In activity up to 6 days after intramyocardial injection. As a positive control of the FISH method, we identified labeled cells both in culture and immediately after cell injection in one pig. This pilot trial suggests that after intramyocardial injection (111)In stays in the myocardium despite possible disappearance of labeled cells. This questions the clinical use of (111)In-labeled cells for tracking. The results further suggest that xenografting of human MSC into porcine hearts leads to inflammation contradicting previous studies implying a special immunoprivileged status for MSC.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">19921546</PMID><DateCreated><Year>2010</Year><Month>03</Month><Day>29</Day></DateCreated><DateCompleted><Year>2010</Year><Month>06</Month><Day>17</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1875-8312</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Mar</Month></PubDate></JournalIssue><Title>The international journal of cardiovascular imaging</Title><ISOAbbreviation>Int J Cardiovasc Imaging</ISOAbbreviation></Journal><ArticleTitle>Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected 111In-labeled human mesenchymal stromal cells.</ArticleTitle><Pagination><MedlinePgn>273-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10554-009-9532-4</ELocationID><Abstract><AbstractText>This pilot trial aimed to investigate the utilization of (111)In-labeling of mesenchymal stromal cells (MSC) for in vivo tracking after intramyocardial transplantation in a xenotransplantation model with gender mismatched cells. Human male MSC were expanded ex vivo and labeled with (111)In-tropolone. Ten female pigs were included. The labeled cells were transplanted intramyocardially using a percutaneous injection system. The (111)In activity was determined using gamma camera imaging. Excised hearts were analyzed by fluorescence in situ hybridization (FISH) and microscopy. Gamma camera imaging revealed focal cardiac (111)In accumulations up to 6 days after injection (N = 4). No MSC could be identified with FISH, and microscopy identified widespread acute inflammation. Focal (111)In accumulation, inflammation but no human MSC were similarly seen in pigs (N = 2) after immunosuppression. A comparable retention of (111)In activity was observed after intramyocardial injection of (111)In-tropolone (without cells) (N = 2), but without sign of myocardial inflammation. Injection of labeled non-viable cells (N = 1) also led to high focal (111)In activity up to 6 days after intramyocardial injection. As a positive control of the FISH method, we identified labeled cells both in culture and immediately after cell injection in one pig. This pilot trial suggests that after intramyocardial injection (111)In stays in the myocardium despite possible disappearance of labeled cells. This questions the clinical use of (111)In-labeled cells for tracking. The results further suggest that xenografting of human MSC into porcine hearts leads to inflammation contradicting previous studies implying a special immunoprivileged status for MSC.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lyngbaek</LastName><ForeName>Stig</ForeName><Initials>S</Initials><Affiliation>Department of Cardiology, The Heart Centre, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.</Affiliation></Author><Author ValidYN="Y"><LastName>Ripa</LastName><ForeName>Rasmus S</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Haack-Sørensen</LastName><ForeName>Mandana</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Cortsen</LastName><ForeName>Annette</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kragh</LastName><ForeName>Linda</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Andersen</LastName><ForeName>Claus B</ForeName><Initials>CB</Initials></Author><Author ValidYN="Y"><LastName>Jørgensen</LastName><ForeName>Erik</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Kjaer</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kastrup</LastName><ForeName>Jens</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Hesse</LastName><ForeName>Birger</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>11</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Int J Cardiovasc Imaging</MedlineTA><NlmUniqueID>100969716</NlmUniqueID><ISSNLinking>1569-5794</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Radiopharmaceuticals</NameOfSubstance></Chemical><Chemical><RegistryNumber>14283-79-5</RegistryNumber><NameOfSubstance>indium tris(tropolonate)</NameOfSubstance></Chemical><Chemical><RegistryNumber>7L6DL16P1T</RegistryNumber><NameOfSubstance>Tropolone</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Chromosomes, Human, Y</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Heart</DescriptorName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Inflammation</DescriptorName><QualifierName MajorTopicYN="N">pathology</QualifierName><QualifierName MajorTopicYN="N">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Injections, Intramuscular</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Mesenchymal Stem Cell Transplantation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mesenchymal Stromal Cells</DescriptorName><QualifierName MajorTopicYN="N">pathology</QualifierName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Myocardium</DescriptorName><QualifierName MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Organometallic Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Pilot Projects</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Radiopharmaceuticals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Swine</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Transplantation, Heterologous</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tropolone</DescriptorName><QualifierName MajorTopicYN="Y">analogs & derivatives</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">X Chromosome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>7</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>10</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2009</Year><Month>11</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>11</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>11</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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